| id | nickname | date | experimental_design | media_used | media_used_concentration (%) | media_mod | additives | additive_concentration (%) | temperature (°C) | duration (hours) | solid_or_liquid_culture | volume_of_liquid_culture (mL) | number_of_plates_used | solvent_used_for_extraction | resin_used | drying_agent_used | mass_of_crude_extract (mg) | num_aa_screen_extract | isolate_id | sample_id | institution |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| id | nickname | date | experimental_design | media_used | media_used_concentration (%) | media_mod | additives | additive_concentration (%) | temperature (°C) | duration (hours) | solid_or_liquid_culture | volume_of_liquid_culture (mL) | number_of_plates_used | solvent_used_for_extraction | resin_used | drying_agent_used | mass_of_crude_extract (mg) | num_aa_screen_extract | isolate_id | sample_id | institution |
| 1016795 | Ext#1_JB5 | 2024/10/09 | Four PDA lawn plates were made using isolate JB5. Lawn plates were inoculated 10/9/2024; incubated for 120 hours between 10/9/2024 and 10/14/2024; sliced into small chunks and frozen in two 50mL conical tubes 10/14/2024 (two plates of agar per tube); then thawed 10/16/2024. Following thaw, agar chunks were soaked in 3:1 ethyl acetate:water solution and centrifuged to move the extracted inhibitory chemical(s) to the ethyl acetate while the primary metabolites remained in the water. Ethyl acetate containing extracted inhibitory chemical(s) were removed from conical tubes using Pasteur pipets, combined in a beaker, dried with sodium sulfate, and then gravity filtered to produce the final solution ideally containing only ethyl acetate and the extracted inhibitory chemical. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 120 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1014668 | 1004610 | Salisbury University | ||||
| 1016900 | Ext#1_JG6 | 2024/10/09 | We cut our agar plates into small square chunks, then froze and thawed them as this helps break up the agar to make extracting the antibacterial chemicals easier. We then mixed the agar chunks with ethyl acetate/water, shook it up, and centrifuged it. Finally, after letting the solution settle, we transferred the top solution off (ethyl acetate layer) and pipetted it into a clean beaker, added some drying solvent (sodium sulfate) to absorb the remaining water in the ethyl acetate layer, and then gravity filtered it into a clean vial. It is now sitting in a fume hood so that the ethyl acetate evaporates. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 120 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1014662 | 1004587 | Salisbury University | ||||
| 1017058 | Ext#1_AG9 | 2024/10/09 | Used thawed agar chunks and mixed them in ethyl acetate and water. Shook The tube around for a few minutes. pipetted the top cleared layer into a beaker. Made a gravity filter and let it drip into a small bottle. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 120 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1015165 | 1004496 | Salisbury University | ||||
| 1017158 | Ext#1_JG17 | 2024/10/09 | Goal of experiment is to extract inhibitory chemical from agar. Grow four spread plates for 120 hours. Chop up agar plates. Freeze tubes completely, that tubes completely. Add 10-15 ml of ethyl acetate and 5-10 ml water. Invert a few times for 10 minutes. Centrifuge-2000 rpm for 10 mins for separate layers. Use a glass Pasteur pipet to transfer top layer to a clean beaker. Add drying agent sodium sulfate to absorb remaining water for 5 minutes. Gravity filter into a pre-weighed vial. Remove ethyl acetate (ideally by roto Vap or let evaporate in fume hood). | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 120 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1015068 | 1004587 | Salisbury University | ||||
| 1017186 | Ext#_CB18 | 2024/10/09 | First we froze, and then thawed our solution. Next we mixed it with ethyl acetate and water. The non-polar antibacterial chemicals moved into the ethyl acetate layer. We place contents in the centrifuge to separate the water and ethyl acetate layers. Finally, after soaking up the rest of the salts, we remove ethyl acetate liquid top layer using a pipet and let it evaporate. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 168 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1015096 | 1004403 | Salisbury University | ||||
| 1017331 | MillerK_F24 | 2024/10/09 | The extraction technique involves breaking down agar chunks that contain antibacterial compounds by chopping them up and placing them in a tube and then freezing and thawing them which facilitates the release of these compounds. The agar is then mixed with ethyl acetate and water, allowing the antibacterial chemicals to partition into the ethyl acetate layer. After mixing and centrifuging the mixture to separate the layers, the ethyl acetate layer is collected. To remove any remaining water, a drying agent (sodium sulfate) is added before gravity filtering the solution into a pre-weighed vial. Finally, the ethyl acetate is evaporated, leaving behind a dried residue of the extracted antibacterial chemicals. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 120 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1015041 | 1004499 | Salisbury University | ||||
| 1017412 | EXT#1_GW8 | 2024/10/09 | Cut up 4 agar plates from isolate GW8 streak. Mixed with ethyl acetate and water, put it in the centrifuge for 2000 rpm for 10 minutes, which separates the layers. We took the liquid and extracted it into a beaker which then we mixed sodium sulfate to absorb the water. After filtering it we remove the ethyl acetate by putting it in the fume hood. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 96 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1017134 | 1004384 | Salisbury University | ||||
| 1017792 | AL1 | 2024/10/09 | Chemical extraction involved mixing Ethyl Acetate into our PDA agar isolate to facilitate antibiotic chemical separation & allow for the antibiotic to mix completely with the Ethyl Acetate. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 120 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1012540 | 1004460 | Salisbury University | ||||
| 1018307 | Ext#1_EM8 | 2024/10/09 | To perform the chemical extraction, we obtained the streak plate of EM 8 (PDA) after it was frozen and thawed. Next, we cut the agar with a microspatula and added them to a tube. We mixed agar chunks with ethyl acetate and water, separated the water and ethyl acetate layers via centrifuging, and removed the ethyl acetate layer and let the ethyl acetate evaporate resulting in all nonpolar chemical being extracted from the agar as dried residue. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 120 | Solid | 4 | ethyl acetate | sodium sulfate | 1 | 1015746 | 1004719 | Salisbury University | ||||
| 1019085 | Ext#1_ML14 | 2024/10/09 | We pipetted the top layer of Ethyl Acetate with our antibiotic chemical out of the tube, leaving behind the agar and water. Then we added Sodium Sulfate and ran it through a paper filter to remove any water left over and then let it dry. | Potato Dextrose Agar (PDA) | 100 | nystatin | 50 | 28 | 120 | Solid | 4 | ethyl acetate | 0 | sodium sulfate | 1 | 1015111 | 1004595 | Salisbury University |
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