| id | nickname | date | experimental_design | media_used | media_used_concentration (%) | media_mod | additives | additive_concentration (%) | temperature (°C) | duration (hours) | isolate_form | eukaryotic_organism | results | eukaryotic_inhibition | isolate_id | sample_id | institution |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| id | nickname | date | experimental_design | media_used | media_used_concentration (%) | media_mod | additives | additive_concentration (%) | temperature (°C) | duration (hours) | isolate_form | eukaryotic_organism | results | eukaryotic_inhibition | isolate_id | sample_id | institution |
| 1001744 | Lake Water 11 | 2024/04/02 | Cut out a slice of agar inoculated with Fusarium sambucinum fungus and circled the fungus slice with bacterial colonies from our library plates, incubating for 5 days at 30C. | Potato Dextrose Agar (PDA) | 100 | none | 30 | 120 | live culture | Fusarium sambucinum (dry rot) | Lake Water 11 showed inhibition of Fusarium sambucinum. | Yes | 1001719 | 1001720 | Madison College | ||
| 1001755 | Lake Water 11 | 2024/03/26 | Cut out a slice of Phytophthora erythroseptica culture and placed on PDA agar, circled the culture with bacterial culture from library plates and incubated at 30C for 5 days. | Potato Dextrose Agar (PDA) | 100 | none | 30 | 120 | live culture | Phytophthora erythroseptica (pink rot) | Lake Water 11 inhibited the growth of the oomycete. | Yes | 1001719 | 1001720 | Madison College | ||
| 1037395 | Radish GW8 | 2024/11/11 | We had 4 water agar plates given to us. Each plate we put 8 radish seeds on all separated with 2 in the top row, 2 in the second, 2 in the third row, and 2 in the bottom. The 2 top radish seeds were left alone with nothing put on them. Our second row got 10 microliters of ethyl acetate. Our third row received 10 microliters of our GW8 extract, and our final row got 10 microliters of nystatin. | Water Agar | 100 | none | 22 | 48 | extract | Raphanus sativus (radish) | Our results showed a good amount of growth. We learned that our extract was not toxic and helped the radish seeds grow. We had about 5 seeds that did not grow, and those were found in either the row with nothing or the row with nystatin. | No | 1017134 | 1004384 | Salisbury University | ||
| 1038566 | Radish CB18 | 2024/11/11 | We placed 3 seeds in 4 different groups on our water agar. We dispensed 5 micro liters onto each seed of different extracts. The first group had nothing dispensed on it, this was our control. The second group had water dispensed on it. Another group had ethyl acetate dispensed on it. Finally, our isolate, which was resuspended in ethyl acetate was dispensed on the last group of seeds. In total, we used 12 seeds, in groups of 3, 4 groups total, with 5 micro liters on each seed. | Water Agar | 100 | none | 0 | 22 | 168 | extract | Raphanus sativus (radish) | -12 total seeds (in groups of 3) -all seeds had growth -conclusion: our extract was not toxic | No | 1015096 | 1004403 | Salisbury University | |
| 1040636 | Radish JB5 | 2024/11/11 | All seeds were placed on water agar plates at 100% concentration with no additional additives. Seeds with positioned with sterilized tweezers, with four seeds in each treatment group so sample size/repetitions = 4. Each plate was tested with a different substance, but the left half of the plates were additionally tested with 5 µL of nystatin to test for growth inhibition with nystatin additive under all conditions. Therefore, each plate contained two treatment groups with four seeds each, so each plate contained eight radish seeds. All treatments were applied directly to seeds by pipette. Plates were assigned letter IDs to make the explanation of the experimental design easier; photos of the plates are attached under "additional notes". Plate A was inoculated with 5 µL of water; plate B was inoculated with 5 µL of ethyl acetate; plate C was inoculated with 2.5 µL of JB5 chemical extract; plate D was inoculated with 5 µL of JB5 chemical extract. JB5 extract was resuspended in 80 µL of ethyl acetate prior to the application of the extract to the seeds. Water (A) and ethyl acetate (B) plates were used as controls to ensure that the seeds would germinate under optimal conditions (with only water added) and to ensure that the ethyl acetate used to resuspend the JB5 chemical extract would not interfere with the germination of the radish seeds. Plates treated with the JB5 chemical extract (C and D) were the experimental groups. | Water Agar | 100 | none | 22 | 48 | extract | Raphanus sativus (radish) | All treatment groups had at least 75% germination of radish seeds. Length of sprouts ("radicals") in each treatment group are as follows in centimeters: Plate A - 5 µL water: 0.1, 1.1, 1.2, 2.1; 5 µL water + 5 µL nystatin: 0 (no germination), 0.8, 1.3, 2.4. Plate B - 5 µL ethyl acetate: 1.1, 1.3, 1.5, 1.7; 5 µL ethyl acetate+ 5 µL nystatin: 0 (no germination), 1.1, 1.2, 1.4. Plate C - 2.5 µL JB5 chemical extract: 0.5, 1.4, 1.8, 1.9; 2.5 µL JB5 chemical extract + 5 µL nystatin: 1.0, 1.1, 1.1, 1.2. Plate D - 2.5 µL JB5 chemical extract: 0.9, 1.0, 1.1, 1.3; 2.5 µL JB5 chemical extract + 5 µL nystatin: 0.2, 0.7, 0.9, 1.1. The average length of sprouts for each treatment group are as follows, with non germinating seeds excluded to account for natural variations in the viability in each seed: Plate A - 5 µL water: 1.125; 5 µL water + 5 µL nystatin: 1.5. Plate B - 5 µL ethyl acetate: 1.4; 5 µL ethyl acetate+ 5 µL nystatin: 1.23. Plate C - 2.5 µL JB5 chemical extract: 1.4; 2.5 µL JB5 chemical extract + 5 µL nystatin: 1.1. Plate D - 2.5 µL JB5 chemical extract: 1.075; 2.5 µL JB5 chemical extract + 5 µL nystatin: 0.725. Photos of each plate are included under "additional notes" section. | No | 1014668 | 1004610 | Salisbury University | ||
| 1042640 | RadishJG6 | 2024/11/11 | We had two controls, water and ethyl acetate, and we had our isolate extract. On the water agar plate we placed 6 seeds, we did this three times, and to two of the seeds we added 10 uL of control 1, then 10 uL of control 2 to two seeds, and finally 10 uL of the isolate extract to two seeds, then repeated this with two more plates. Although, with the third plate I ran out of my isolate extract so I only used 1 seed on that plate for the isolate extract, meaning in total I had 5 seeds for this part. The chemical extract that was resuspended in ethyl acetate was isolate JG6. We then placed our plates in a drawer and after 48 hours we checked the growth and then again after 1 week. | Water Agar | 100 | none | 22 | 168 | extract | Raphanus sativus (radish) | We used 6 seeds of each control and then 5 of the isolate. All of the controls seeds had growth besides one seed with control 1 on it on plate 3. For my isolate I had growth on 4 out of 5 of the seeds and the one seed that did no grow was on plate 3. All of the seeds growth look very healthy after 48 hours and 168 hours. Each seed continued to grow over time. In conclusion, my extract was not toxic. | No | 1014662 | 1004587 | Salisbury University | ||
| 1042804 | RadishJG17 | 2024/11/11 | To inoculate each seed, we had a control group which was just water on the seed. We tested the control at 10 microliters, 20 microliters, and 30 microliters. Our extract JG17 was placed on the other seed on each plate at 10 microliters, 20 microliters, and 30 microliters. Each treatment on the 3 plates consisted of 2 seeds. One being the control treatment and the other being our extract. The chemical extracts were respended in ethyl acetate before placing the treatment onto the seeds. There were no replicates in our experiment just testing 3 different amounts of extract. The experiment was conducted over the course of a week. We collected results at each class period after 2 days and then after 7 days. To measure the impact that our extract has on the radish seeds we measured the impact through how much the radish seed sprouts in each condition by inches. | Water Agar | 100 | none | 23 | 48 | extract | Raphanus sativus (radish) | 10 microliters extract- 1/2 inch growth, fuzzy 10 microliters water-1/2 inch growth, fuzzy 20 microliters extract- 0.6 inch little over 1/2 inch 20 microliters water-0.6 inch little over 1/2 inch 30 microliters extract-1/4 inch wrapped around seed 30 microliters water- 1/4 inch wrapped around seed In each of the conditions the control and extract on the radish seed, the seed grew about the same amount. | No | 1015068 | 1004587 | Salisbury University | ||
| 1042846 | RadishJG17 | 2024/11/11 | To inoculate each seed, we had a control group which was just water on the seed. We tested the control at 10 microliters, 20 microliters, and 30 microliters. Our extract JG17 was placed on the other seed on each plate at 10 microliters, 20 microliters, and 30 microliters. Each treatment on the 3 plates consisted of 2 seeds. One being the control treatment and the other being our extract. The chemical extracts were respended in ethyl acetate before placing the treatment onto the seeds. There were no replicates in our experiment just testing 3 different amounts of extract. The experiment was conducted over the course of a week. We collected results at each class period after 2 days and then after 7 days. To measure the impact that our extract has on the radish seeds we measured the impact through how much the radish seed sprouts in each condition by inches. | Water Agar | 100 | none | 23 | 168 | extract | Raphanus sativus (radish) | 10 microliters extract- 1 1/4 inch seed growth ,2 sprouts of it 10 microliters water-3 1/2 inch seed growth 20 microliters extract- 3 1/2 inch seed growth, 3 sprouts of it 20 microliters water-3-inch seed growth, 2 sprouts 30 microliters extract-3 1/2 inch seed growth 30 microliters water- 3 1/4 inch seed growth In each of the conditions the control and extract on the radish seed, the seed grew about the same amount. | No | 1015068 | 1004587 | Salisbury University | ||
| 1043324 | Radish (EM8) | 2024/11/11 | Before performing the experiment, I performed a chemical extraction and resuspended my extract in ethyl acetate. I used a total of 4 water agar plates and 16 radish seeds. I placed 4 radish seeds on each water agar plate. I labeled each plate. On plate one, I covered each radish seeds with 10 microliters of water using a micropipet and distilled water. On plate two, I covered each radish seed with 10 microliters of ethyl acetate using a micropipet. One plate three, I covered each radish seed with 10 microliters of my extract using a micropipet. On plate four, I covered each radish seed with 5 microliters of my extract and 5 microliters of ethyl acetate using a micropipet. I then incubated all four plates at room temperature for 7 days (168 hours) and examined the results. | Water Agar | 100 | none | 22 | 168 | extract | Raphanus sativus (radish) | The growth of the radish seeds was not inhibited on plates one, two, three, or four. The radish seeds were able to grow substantially. The amount of growth of the radish seeds was extremely similar on all four plates. On plate one, all four radish seeds grew more than two inches. On plate two, all four radish seeds grew more than two inches. On plate three, all four radish seeds grew more than two inches. On plate four, all four radish seeds grew more than two inches. My results showed that my extract was not toxic to radish seeds, indicating that it is not toxic to eukaryotic cells and may be beneficial in future clinical use. | No | 1015746 | 1004719 | Salisbury University | ||
| 1043857 | MillerK_F24 | 2024/11/20 | Each seed except the control was inoculated with 10 μL of each liquid. We used 4 plates and each plate had 5 seeds. We used extract, water, or nystatin for each plate except for the control which we did not inoculate with any liquid. After 24 hours and 120 we measured the length of the radish seed stems to measure the growth. | Water Agar | 100 | none | 22 | 120 | extract | Raphanus sativus (radish) | Measurements after 24 hours Extract plate: 0.8 cm, 0.9 cm, 0.7 cm, 0.8 cm, 0 cm Water plate: 1.8 cm, 1.1 cm, 1.5 cm, 1.5 cm, 0 cm Nystatin plate: 1.5 cm, 1.4 cm, 1.0 cm, 1.2 cm, 0 cm Control plate: 1.6 cm, 2.5 cm, 1.5 cm, 0 cm, 0 cm Measurements after 120 hours Extract plate: 12.5 cm, 14.0 cm, 15.0 cm, 10.2 cm, 8.3 cm Water plate: 12.4 cm, 15.0 cm, 16.0 cm, 13.2 cm, 0 cm Nystatin plate: 14.3 cm, 11.2 cm, 12.3 cm, 7.5 cm, 0 cm Control plate: 10.6 cm, 9.5 cm, 12.0 cm, 11.0 cm, 3.0 cm | No | 1015041 | 1004499 | Salisbury University |
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